Variant detection for pooled sequence data (CRISP) | Scripps Translational Science Institute

This figure, published in Dr. Bansal’s 2010 Bioinformatics paper, illustrates how comparing allele counts across multiple DNA pools can be used to distinguish rare variants from sequencing errors.

CRISP is a software program designed by Vikas Bansal, Ph.D., to detect SNPs and short indels from pooled sequencing data. CRISP is designed to detect both rare and common variants by utilizing sequence reads from next-generation sequencing of multiple DNA pools.

For more information:

Efficient and cost efective population resequencing by pooling and in-solution hybridization.Bansal V, Tewhey R, LeProust EM, Schork NJ. PLoS ONE 6(3): e18353.2011 March 30. PMID: 21479135.
A statistical method for the detection of variants from next-generation resequencing of DNA pools. Bansal V. Bioinformatics. 26(12): i318-i324. 2010. PMID: 20529923.

Infrastructure

CRISP